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Introduction to the course. Examples of online portfolios by students and professionals. (You will be submitting an online portfolio at the end of this course.)

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Examples of online design: fonts and typography. Web constraints for font families; best practices in CSS.
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Drawing, symbols, and simple animation in Flash. Lessons 1 and 2 (book).
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Flash Timeline, frames, symbols, the Motion Editor. Examples of animation in news packages. Lesson 4 (book).
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Use of Movie Clip Symbols to embed complex functionality in Flash. Sliding panels. Pop-up information panels. Planning a news package; working with maps.
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Working with dynamic text, components, and XML (not covered in the book) in Flash; the XML object (AS3). Lesson 8 (book). Loading external images and text into Flash.
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Modular Flash packages: Loaded SWFs (and masks); the Loader object (AS3). Also: Work on your Flash Project. Lesson 9 (book).
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Flash on Web pages: Positioning Flash movies with CSS; Flash version detection; demystifying the Flash object parameters. SWFObject 2.0. The Bandwidth Profiler. Lesson 10 (book).
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To confirm immune-mediated antitumor activity in the sildenafil-treated groups, we performed an in vivo cytotoxicity assay. BALB/c mice were injected with PBS (naive), vaccinated with -irradiated CT26WT or C26GM, or challenged with CT26WT or C26GM tumors on day 0. On day 12 (CT26WT) or day 5 (C26GM), all mice received carboxyfluorescein diacetate succinimidyl ester (CFSE)–labeled splenocytes pulsed with the MHC class I–restricted AH-1 peptide (CFSEhigh), admixed with CFSE-labeled splenocytes pulsed with the irrelevant hemagglutinin (HA)-peptide (CFSElow). In vivo T cell cytotoxicity was determined 40 h later (Fig. 2). These time points were chosen based on the kinetics of tumor outgrowth observed in Fig. 1 A and Fig. 1 B, respectively, when tumor size significantly differed between untreated and sildenafil-treated mice. As expected, an endogenous AH1-specific immune response was observed in the vaccinated mice as compared with their tumor-bearing counterparts. PDE5 inhibition in the vaccine-primed mice failed to augment antigen-specific CD8 responsiveness compared with no treatment. In contrast, tumor-bearing mice treated with sildenafil early after tumor challenge generated antigen-specific immunity that was significantly greater then that observed in their untreated counterparts and similar, or even superior, to that induced by vaccination. Collectively, this is the first indication that PDE5 inhibitors can modulate antitumor immunity. Because the sildenafil-mediated antitumor immune response does not completely eradicate tumors, tumor escape mechanisms may be associated with their outgrowth. To test this hypothesis, the parental CT26WT cell line, as well as the CT26 tumor removed on day 24 from sildenafil-treated mice (either AH-1 pulsed or unpulsed), and BALB/c splenocytes were incubated with either AH-1 peptide-primed (Fig. S1 C) or tumor-primed (Fig. S1 D) effector T cells. Although effector T cells recognized the parental CT26WT line and released IFN- in the assay, they failed to recognize the sildenafil-derived tumor. Its recognition, however, was restored by loading the sildenafil-derived tumor with the AH-1 peptide. (Fig. S1, C and D). These results suggest that the immune response in sildenafil-treated mice does not result in complete tumor eradication but rather in the selection of antigen-escape variants. When the penis is inflated, the prosthesis makes the penis stiff and thick, similar to a natural erection. Most men rate the erection as shorter than their normal erection; however, newer models have cylinders that may increase the length, thickness, and stiffness of the penis. Stop smoking. * Make you partner(s) go crazy with lust for your newfound abilities and appearance. There are no formal tests to diagnose erectile dysfunction. 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